I'm not sure I agree a hundred percent on your police work there, @Pat271 .
For one thing, there's no reason that a group of men - related by blood or not - can't consider themselves a "family." Believe me, it won't go down well in San Francisco to suggest otherwise. But more importantly, Airbnb's Nondiscrimination policy prohibits declining or cancelling a booking based on the guests' gender, age, or familial status. So if you're going to accept a booking for 8 people, you don't get to require that some of them be children, female, and/or related to each other. That's a discrimination trifecta.
Any suspicion of discrimination can get a new listing shut down much faster than an established one like yours, so it would be very risky for this host to take your advice if she's invested in a future on Airbnb.
@Lorrie40 this is the steep incline of your learning curve. Of course you can remind the guests of your "no parties" rule, but it doesn't really mean anything because nobody knows what your idea of a party is. The best suggestion I can offer is that you apologetically tell your guests that, as a brand new host, you published your listing before your House Rules were ready for Prime Time, and then provide them with an updated version that goes into specifics. Policies on unregistered visitors, noise, checkout and trash removal, and safety requirements for your boating equipment would be appropriate here. Reminders that your home is CCTV monitored for security would also be appropriate. Reminders of your sleeping arrangements would also be fine. Stereotypical assumptions about what a "guys weekend" means based on your husband's misadventures would be extremely inappropriate, so just don't go there.
If the booking is still within the free cancellation grace period, you can advise the guests that if the amended rules aren't a good fit for their plans, they can still get out of the booking. Maybe they'll take the hint and reconsider. But I hope you also reconsider your occupancy rate - there's no reason you have to allow the absolute maximum number of people just because you can. I don't really see how the sleeping arrangements would work for 8 people, anyway - you describe it as "1 King, 1 Full, 1 Bunk with twin/full bed combo" - why would you host more than 6?