@Kim-and-Jen0
"Do you think it's rude to print out the houserules and post it in the room? I do not want to offend anyone. ;)"
Put yourself in the mental position that you are running a business, maybe that helps?
Your house, your rules. You can hang up your rules or put them in a binder with informative information like Wifi code as a house manual in their room, Just remember guests often don't read.
Which is why you will have to tell them the most important things verbally. like it or not.
If your guests are still staying longer, you'll have to approach them (the wife) and say something like:
Jenny, I've cleared your own space in the cupboard in the kitchen, so you don't need to search for free space.
Jenny, I noticed you were maybe looking for something in ..... How can I help you?
Your listing does say you share your home, so for less sensitive and encroaching guests that's an invitation to take over your whole home!
You'll absolutely need to create boundaries. And again when giving the tour tell them what you offer and what you don't do or what's off limits. Just make it sound like everything you offer is a positive, and don't make things you don't share (office, private bedroom) as a negative. You'll find yourself sooner or later either installing locks or putting a 'Private' (or No Entry) sign where needed. Absolutely no problem.
Clarity. Lots of it.