Hi @Rosalind9 , welcome to hosting, what a great place your parents left you! It's probably a good thing that this happened at the beginning, so you can adjust your house rules and your overall approach.
Obviously, it's a difference if you have 12 people at the house (your max) or just one person. I would suggest to have a new set of towels per person available after two days. Best is to adjust it to how many days and how many people you will be having, and do the math. A lot also depends if you expect the guests to do the laundry for themselves or if you will take care of it.
the longer the stay, the clearer you need to be in what you expect guests to participate in, such as taking out the trash, changing the linens after 4 or 5 days, keeping the kitchen clean, etc. Best is actually to have your go to person come in every 4 or 5 days and do a general cleaning. - That way you will know what is going on and can stay on top of it.
Bottomline is that you need to spell out everything and be very clear about it, not necessarily in your house rules, but have it posted in the house, in the kitchen, bathroom, wherever. Don't rely on people to share your ideas of what is "reasonable", u tomyou tomdefine it for them.
While you are rethinking all that, I would also go back into your listings and define clearly what is available and what is not, like your two listings look exactly the same, but one is max 12 people, one (room only) for 2 people. The link to a website you put in the second listing (room only) for space description doesn't work - you cannot put any links to other sites into your listing. so do 2 people who rent the room have the run of the whole house?Lots to clarify....
Also, I see you have a cancellation as your first "review": read up on what cancellations mean to hosts - you don't ever want to do that, unless absolutely necessary.