@Stephanie - @Gordon0's comment says it ALL! I think the most important thing is putting yourself in the shoes of guests when you're reviewing your space. What are they going to see when they walk in? What will confuse or annoy them? What will charm them?
Since I can't be at my place to prepare for guests, I rely on my housekeeper, who luckily is as motivated as I am to make everything perfect. (She even reads all the reviews after guests check out!) When I am there we go over the linens, the kitchen organization, the bedrooms, really everything, together, and talk about how guests are using things and what more they might need. And she knows that when I say "This looks sketchy - let's get rid of it," I had better never see that thing again.
My obsessions are order in every cabinet, closet, and drawer and level surfaces in every room that are free of clutter. Like Gordon I cannot ABIDE plastic or wire hangers ("SKETCHY!!!"), nor can I bear the previous guests' dog-eared travel brochures (EVEN SKETCHIER! And my previous housekeeper always saved them!).
A recent post noted that you should remove every single personal item, but I think guests like some touches - here are two photographs of my family house that show some of my children's artwork framed and hung on the walls. I believe guests with families like this kind of touch because it makes them less anxious about their children touching anything.